iq sybr green supermix with opticon  (Bio-Rad)

Journal: Frontiers in Physiology

Article Title: Tangshen Formula Attenuates Diabetic Nephropathy by Promoting ABCA1-Mediated Renal Cholesterol Efflux in db/db Mice

doi: 10.3389/fphys.2018.00343

Figure Lengend Snippet: List of primers used for quantitative real-time PCR.

Article Snippet: Total RNA was extracted from renal tissues and cultured cells and purified using an RNeasy kit according to the manufacturer's instructions (Qiagen, Valencia, CA, USA), and real-time PCR was performed with an Opticon real-time PCR machine (Opticon 2, Bio-Rad Labs., Hercules, CA, USA) using the IQ SYBR Green Supermix reagent (Bio-Rad Labs., Hercules, CA, USA) as previously described (You et al., ).

Techniques: